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Description

The electroporation cuvette was inserted into the Gene Pulser Xcell Total System (Bio-Rad, Hercules, CA, USA) and electroporated under the following conditions: pulse type: square waveforms

This converts another ROS, superoxide (O2-), into hydrogen peroxide

Alternatively, the yeast may be grown under continuous culture conditions, allowing for continuous harvesting of culture medium and therefore recovery of secreted glutathione