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Signal intensities of HJURP or EGFP-mAid HJURP on the blot were measured with normalization to -tubulin intensity in each blot

Quantification of ATM expression Cultured hair follicle melanocytes were seeded into 8-well chamber slides (~ 10,000 cells/well) and left to attach for at least 24 h, fixed in ice-cold methanol for 10 min, and incubated with blocking buffer of 10% donkey serum (Sigma-Aldrich, UK) for at least 30 min

Why Add Vitamin C

And Th2 cells also play a crucial role in allergic responses, as they encourage B cell activation, differentiation, proliferation, and class switch recombination, leading to the production of allergen-specific IgE antibodies