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Liver tissue health has mildly improved in the groups receiving GSH treatment, and VCO treatment

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A significantly decreased MDA level following riboflavin therapy has also been reported in experimental models of several other diseases such as acute lung injury (Reference Al-Harbi, Imam and Nadeem22) , hepatotoxicity (Reference Al-Harbi, Imam and Nadeem25,Reference Bashandy, Ebaid and Moussa26) , diabetes (Reference Alam, Iqbal and Naseem31) and cisplatin- (a chemotherapy medication) induced toxicities (Reference Naseem, Hassan and Alhazza28,Reference Salman and Naseem29)

Fluorescence of CM-H 2 DCFDA dye was used as an indicator of the presence of intracellular ROS, and was measured over a period of 24 h, the minimum time shown for autophagy induction to occur (Supplementary Figure S3)