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Materials and Methods Chemicals All chemicals and reagents used in this study were of molecular and analytical grade and were purchased from Sigma Chemical Co

Vitamin B12 deficiency is most common in adults over 50, vegans and vegetarians, pregnant women, and those with chronic health conditions like Crohns disease, inflammatory bowel disease (IBS), or rheumatoid arthritis

For GI applications, some practitioners recommend oral administration to maximize local gastrointestinal exposure

if you expose it to liquid too forcefully or shake it violently, you risk damaging the molecular structure (denaturation), which can undermine research quality