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Standard GI research endpoints include: macroscopic damage scoring (CDAI-equivalent scales in IBD models), histological assessment (H&E staining, villus/crypt morphometry), tight junction protein expression (Western blot, immunofluorescence), permeability assays (FITC-dextran, Ussing chambers), and inflammatory cytokine panels

When managing patients taking opioid analgesics, particularly those who have been treated for an extended period of time and/or with high doses for chronic pain, ensure that a multimodal approach to pain management, including mental health support (if needed), is in place prior to initiating an opioid analgesic taper

Mitochondria, rich in polyunsaturated phospholipids and ROS, have also been highlighted, particularly under metabolic stress 23

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