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The quantification of GSH was carried out by comparing the fluorescence produced by the samples versus that of a standard solutions curve with known concentrations of GSH.11 To measure GSSG concentration, 25L aliquots of saliva were incubated with 10L of a N-ethylmaleimide solution (NEM, 5mg/mL in distilled water) for 40min at room temperature

doi: 10.1186/s12885-020-07067-x 153 RenDLGhoorunRAWuXHChenHLZhouQWuXB

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(3) Nanotherapy for ferroptosis in ccRCC With the advancement of materials science, an increasing number of studies have found that nanoparticles can induce ferroptosis in various tumor cells including lung cancer, breast cancer, liver cancer, and others (264)