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However, while uIL-18 shows higher predictive efficacy in pediatric cohorts, the significant variability in reported cut-off values, ranging from 6.39 to 125 pg/ml, underscores the need for standardized thresholds in clinical practice [236]

Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature